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dr gfp reporter plasmid  (Addgene inc)


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    Addgene inc dr gfp reporter plasmid
    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme <t>of</t> <t>DR-GFP</t> reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells <t>were</t> <t>transfected</t> with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
    Dr Gfp Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reporter+plasmid+dr+gfp/pDRGFP+(Plasmid+%2326475)/bio_rxiv__64898__2026__02__17__706442-227-6-9
    Average 96 stars, based on 188 article reviews
    dr gfp reporter plasmid - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "STING safeguards epithelial genome integrity and protects from carcinogenesis via mitotic checkpoint control"

    Article Title: STING safeguards epithelial genome integrity and protects from carcinogenesis via mitotic checkpoint control

    Journal: bioRxiv

    doi: 10.64898/2026.02.17.706442

    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme of DR-GFP reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells were transfected with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
    Figure Legend Snippet: a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme of DR-GFP reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells were transfected with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.

    Techniques Used: Western Blot, Single Cell Gel Electrophoresis, Two Tailed Test, Reporter Assay, Transfection, Control, Immunoprecipitation, Co-Immunoprecipitation Assay, Protein Kinase Assay, Protein Extraction, Activity Assay, Kinase Assay, Transformation Assay, Fluorescence, Phospho-proteomics

    Related Articles

    Non-Homologous End Joining:

    Article Title: PC4-mediated Ku complex PARylation facilitates NHEJ-dependent DNA damage repair
    Article Snippet: .. NHEJ reporter plasmid EJ5-GFP (Addgene plasmid #44026) or HR reporter plasmid DR-GFP (Addgene plasmid #26475) was transfected into Huh7 cells respectively. ..

    Article Title: Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity.
    Article Snippet: .. In brief, NHEJ reporter plasmid pimEJ5GFP (Addgene, #44026, USA) or HR reporter plasmid DR-GFP (Addgene, #26475) was transfected in HEK 293T cells. ..

    Article Title: Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity
    Article Snippet: .. In brief, NHEJ reporter plasmid pimEJ5GFP (Addgene, #44026, USA) or HR reporter plasmid DR-GFP (Addgene, #26475) was transfected in HEK 293T cells. ..

    Article Title: CRlSPR/Cas9 screening revealed BlRC6-AS1 /BlRC6 mediates abiraterone resistance via NHEJ pathway-dependent A20 degradation in prostate cancer
    Article Snippet: .. Briefly, The NHEJ reporter plasmid pEJ5-GFP (Addgene, Cat# 44026) or HR reporter plasmid DR-GFP (Addgene, Cat# 26475) was transfected into DU145 and PC3 cells, followed by selection of stable clones with 1 μg /ml puromycin. ..

    Article Title: PC4-mediated Ku complex PARylation facilitates NHEJ-dependent DNA damage repair.
    Article Snippet: .. NHEJ reporter plasmid EJ5-GFP (Addgene plasmid #44026) or HR reporter plasmid DR-GFP (Addgene plasmid #26475) was transfected into Huh7 cells respectively. ..

    Plasmid Preparation:

    Article Title: PC4-mediated Ku complex PARylation facilitates NHEJ-dependent DNA damage repair
    Article Snippet: .. NHEJ reporter plasmid EJ5-GFP (Addgene plasmid #44026) or HR reporter plasmid DR-GFP (Addgene plasmid #26475) was transfected into Huh7 cells respectively. ..

    Article Title: Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity.
    Article Snippet: .. In brief, NHEJ reporter plasmid pimEJ5GFP (Addgene, #44026, USA) or HR reporter plasmid DR-GFP (Addgene, #26475) was transfected in HEK 293T cells. ..

    Article Title: Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity
    Article Snippet: .. In brief, NHEJ reporter plasmid pimEJ5GFP (Addgene, #44026, USA) or HR reporter plasmid DR-GFP (Addgene, #26475) was transfected in HEK 293T cells. ..

    Article Title: CRlSPR/Cas9 screening revealed BlRC6-AS1 /BlRC6 mediates abiraterone resistance via NHEJ pathway-dependent A20 degradation in prostate cancer
    Article Snippet: .. Briefly, The NHEJ reporter plasmid pEJ5-GFP (Addgene, Cat# 44026) or HR reporter plasmid DR-GFP (Addgene, Cat# 26475) was transfected into DU145 and PC3 cells, followed by selection of stable clones with 1 μg /ml puromycin. ..

    Article Title: PC4-mediated Ku complex PARylation facilitates NHEJ-dependent DNA damage repair.
    Article Snippet: .. NHEJ reporter plasmid EJ5-GFP (Addgene plasmid #44026) or HR reporter plasmid DR-GFP (Addgene plasmid #26475) was transfected into Huh7 cells respectively. ..

    Transfection:

    Article Title: PC4-mediated Ku complex PARylation facilitates NHEJ-dependent DNA damage repair
    Article Snippet: .. NHEJ reporter plasmid EJ5-GFP (Addgene plasmid #44026) or HR reporter plasmid DR-GFP (Addgene plasmid #26475) was transfected into Huh7 cells respectively. ..

    Article Title: Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity.
    Article Snippet: .. In brief, NHEJ reporter plasmid pimEJ5GFP (Addgene, #44026, USA) or HR reporter plasmid DR-GFP (Addgene, #26475) was transfected in HEK 293T cells. ..

    Article Title: Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity
    Article Snippet: .. In brief, NHEJ reporter plasmid pimEJ5GFP (Addgene, #44026, USA) or HR reporter plasmid DR-GFP (Addgene, #26475) was transfected in HEK 293T cells. ..

    Article Title: CRlSPR/Cas9 screening revealed BlRC6-AS1 /BlRC6 mediates abiraterone resistance via NHEJ pathway-dependent A20 degradation in prostate cancer
    Article Snippet: .. Briefly, The NHEJ reporter plasmid pEJ5-GFP (Addgene, Cat# 44026) or HR reporter plasmid DR-GFP (Addgene, Cat# 26475) was transfected into DU145 and PC3 cells, followed by selection of stable clones with 1 μg /ml puromycin. ..

    Article Title: PC4-mediated Ku complex PARylation facilitates NHEJ-dependent DNA damage repair.
    Article Snippet: .. NHEJ reporter plasmid EJ5-GFP (Addgene plasmid #44026) or HR reporter plasmid DR-GFP (Addgene plasmid #26475) was transfected into Huh7 cells respectively. ..

    Selection:

    Article Title: CRlSPR/Cas9 screening revealed BlRC6-AS1 /BlRC6 mediates abiraterone resistance via NHEJ pathway-dependent A20 degradation in prostate cancer
    Article Snippet: .. Briefly, The NHEJ reporter plasmid pEJ5-GFP (Addgene, Cat# 44026) or HR reporter plasmid DR-GFP (Addgene, Cat# 26475) was transfected into DU145 and PC3 cells, followed by selection of stable clones with 1 μg /ml puromycin. ..

    Clone Assay:

    Article Title: CRlSPR/Cas9 screening revealed BlRC6-AS1 /BlRC6 mediates abiraterone resistance via NHEJ pathway-dependent A20 degradation in prostate cancer
    Article Snippet: .. Briefly, The NHEJ reporter plasmid pEJ5-GFP (Addgene, Cat# 44026) or HR reporter plasmid DR-GFP (Addgene, Cat# 26475) was transfected into DU145 and PC3 cells, followed by selection of stable clones with 1 μg /ml puromycin. ..



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    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme <t>of</t> <t>DR-GFP</t> reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells <t>were</t> <t>transfected</t> with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
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    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme <t>of</t> <t>DR-GFP</t> reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells <t>were</t> <t>transfected</t> with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.
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    a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme of DR-GFP reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells were transfected with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.

    Journal: bioRxiv

    Article Title: STING safeguards epithelial genome integrity and protects from carcinogenesis via mitotic checkpoint control

    doi: 10.64898/2026.02.17.706442

    Figure Lengend Snippet: a : Western blot analysis of γH2AX from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. b-c : Representative picture (b) and statistical analysis of tail length (c) measured from comet assay from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. Significance was determined using unpaired two-tailed student’s test. d : Western blot analysis of ATM from MODE-K WT and STING -/- cell lines after treated with 2.5μM AraC for 20h. e-f : Scheme of DR-GFP reporter assay ( e ). HR levels of MODE-K WT and STING -/- cell lines were measured by FACS ( f ). Significance was determined using unpaired two-tailed student’s test. g : MODE-K WT and STING -/- cells were transfected with either siRNA against Wip1 or control siRNA for 24h then stimulted with 2.5μM AraC for 20h. Protein levels were measured by western blot assay. h : Co-immunoprecipitation (co-IP) analysis of the interaction between ATM and NBS1 from MODE-K WT and STING-/- cell lines after treated with 2.5μM AraC for 20h. ATM-containing complexes were immunoprecipitated using an anti-ATM antibody and probed for NBS1 by immunoblotting. i : Scheme of protein kinase assay. WT and STING cell lines were treated with 2.5 μM AraC for 20 hours. Following protein extraction, serine/threonine kinase activity and tyrosine kinase activities were analyzed using the Pamgene kinase assay. j : Box plots showing the sum of log2-transformed fluorescence signal intensities of serine/threonine-kinase (STK) phosphorylation levels on the chip. k : Deeper look into different kinase families that are affected by STING deficiency, full figure shown in . l : Deeper look into the results from upstream kinase analysis showing the main affected STK, the full figure shown in . m : MODE-K WT, STING -/- , and cGAS -/- cells were treated with 2.5 μM AraC for 20h. Protein levels were analyzed by western blot. For all the significance analysis: ns = not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.

    Article Snippet: Cells were transiently transfected with the DR-GFP reporter plasmid (Addgene, #26475) using Lipofectamine TM 3000 Reagent (Thermofisher, L3000015) according to the manufacturer’s instructions.

    Techniques: Western Blot, Single Cell Gel Electrophoresis, Two Tailed Test, Reporter Assay, Transfection, Control, Immunoprecipitation, Co-Immunoprecipitation Assay, Protein Kinase Assay, Protein Extraction, Activity Assay, Kinase Assay, Transformation Assay, Fluorescence, Phospho-proteomics